Pictures for the vitro vents requested.
Hello all, after reading the comments in my last post, I have decided to explain the different components of the media and I hope that everyone will understand better why the different components must be added.
The composition of the plant media is important for the plant media to grow properly. Plant tissue is usually made up of the following components: macronutrients, micronutrient, vitamins, amino acid or other nitrogen supplements, sugar(s), other undefined organic supplements, solidifying agents or support systems and growth regulators. For this post, I will be touching on macronutrients, micronutrients.
The media formulation that is used- Murashige and Skoog formulation contains the macronutrients and micronutrients. Macronutrients provide 6 major elements needed for plant growth: Nitrogen, Phosphorous, Potassium, Calcium, Magnesium and Sulfur. 25-60 mM of inoraganic nitrogen is needed in a culture for adequate plant growth. Potassium is needed for cell growth of plant species, most media have potassium in the nitrate, sulfate or choride form at concentration of 20-30 mM. The rest of the elements: Phosphorous, Magnesium, Sulfur and Calcium are included at concentrations ranging from 1-3 mM. Higher concentrations of these nutrients may be needed if dificiency of other nutrients are present. Micronutrients are elements such as Iron, Manganese, Zinc, Boron, Copper and Molybdenum. Chelated form of iron and zinc are commonly used, with Iron being very important. Iron citrate maybe used in culture media but the compounds are had to dissolve and usually precipitate out after media is done. Murashige and Skoog uses ethylene diaminetetraacetic acid-iron chelate to overcome this problem. Sodium and Chlorine are also used in some medium but are not essential for growth. Iron and Molybdenum are added at concentrations of 1 microM, Zinc at 5-30 microM, Manganese at 20-90 microM, Boron at 25-100 microM and Copper at 0.1 microM