Heya guys, hope you all have been enjoying your SIP because i have. I have been attached to the Pilot Food Catering Plant and have started my SIP this week. I am sorry i cannot share any recipes with you guys because it is a trade secret .
There are many jobs to do in a food technology plant. You will have to help the chefs prepare ingredients, perform baking of pastries, grinding of cashew nuts, labeling of food containers, sieving of flour, cracking off eggs, packaging of cakes, maintenance of machines, cleaning the facilities and greasing the baking trays. And this is only the 1st week of my attachment there!!
The preparation of ingredients is very important in baking a premium pastry or cake. Hence it is very imporant to get your measurement units right. A kilogram does not equate to a gram of sugar. Imagine adding a kilogram of sugar into your pastry... YUmYUm.
After the baking proccess and the finished product is achieved(hopefully it turns out well), the TSO and i will have a food tasting session. We will sample the quality of the food and a checklist will be evalutaed. The checklist consist of gradings from 1 to 5 and is very important to gauge the quality of the food before it can be moved to Bristol to be sold. These checklists will not be only given to us, but also to the rest of the students from the Food science and culinery courses for their remarks. Basically the checklists will ask questions on the texture (soft, hard), taste (sour, bitter, sweet, sour), colour (pale, dark, brown), enjoyment (enjoyed eating, feel like puking) and further remarks on how i can be improved. All the checklists wil be gathered and keyed into the computer (not LIS) and tabulated using Excel to find the average response to the food. If the average pertains to liking the product (majority), the cakes/pastrieswill be packaged and labeled before shipped across to the Bristol to be sold.
My Mp involes developing a protocl for zymogram and i briefly explain the principles of a zymogram.
Zymography is a type of electrophoresis technique using SDS-PAGE. It is different from the usual electrophoresis, as it is copolymerised with a substrate (usually geltain or caesin) Why this 2 substrates? Most proteases and periplamic proteins are able to digest there 2 substrates and this allows us to measure and detect the enzyme activity. Another difference for zymogram and normal electrophoresis is that the sample buffer is prepared without boiling and adding of reducing agent such as B-mecapethanol. This is because we do not want to denature the protease and periplasmic proteins as we want them to breakdown the substrate to detct the enzyme activity. After electrophoresis, Triton X-100 is added for the renaturation process and incubated in the digestio buffer for the reaction to take place.Later the zymogram is stained with coomassie blue stain and will show up as clearings/halos whereby there is digestion of periplasmic proteins to the substrate have taken place.
Thanks for reading my entry, have a great MP/SIP ahead
From: Benjamin MA