Hello all, its been 5 since SIP/MP started and it was great seeing you all on friday again. This week it is my turn to share my SIP/MP experience to you all.
I am attached to a plant biotechnology lab, and under the TSO, I help in making plant media. I make two kinds of plant media. One has activated charcoal in it and one does not. The steps to making the plant media are similar, therefore I shall explain one, and list the constituents of the other.
Preperation of tissue plant media
1.) Fill up two 3Litre beakers to over 2.5L with MilliQ water.*
2.) Place the beakers on seperate magnetic stirrers.
3.) Add the constituents in the following order after weighing them/determining their volume in each beaker. (MS media formulation (Murashige and Skoog)-6.45g; Myo-30ml; Organic elements-30ml sucrose 60g).*
4.) Top up the water until both beakers are 3L full.
5.) Next the pH is adjusted to a range of 5.2 to 5.21 using the pH meter
6.) Activated charcoal(1.5g) is added next and allowed to be distributed in the media.**
7.) Next 30 vitro vents(containers that will carry the media) are prepared.
8.) 1.6grams of agar powder are then poured into each vitro vent.
9.) Next a marked measuring cylinder specially used to measure for the volume needed for each vitro vent is used and the media is poured to fill up all the 30 vitro vents.
10.) The vitro vents are then covered with a cover and autoclaved.
Constituents for second set of plant media
1.) MS 6.45g
2.) Myo 30ml
3.) Organic Elements
4.) Sucrose 30g
5.) pH 5.2-5.21
30 vitro vents
*Avoid parallax error by placing the eye onthe same level as the meniscus and read the bottom of the meniscus
**The activated charcoal does not dissolve into the media, therefore it must be thoroughly in the media before it is poured into the vitro vents. And after half the beaker is emptied, place the beaker back on the magnetic stirrer to redistrubute all the activated charcoal that has settled down.
I hope my explanation is clear and easy to understand. If there are any questions, please feel free to ask me. Thanks for taking the time to go through this post.