Hi all! Its been a long time since we've met, hope u guys are having fun in your respective attachments ^_^ Well this is my experience for this week
For this post I will be touching up on Carbon (energy source), Vitamins and Amino Acids or Other Nitrogen Supplements.
The carbohydrate that is used in the plant media that I am making is sucrose, theoretically, glucose and fructose can be substituted in some cases. Sucrose concentration of cell media is usually 2-3%. Carbohydrates are important in the media as very few plant cell lines can supply their own carbohydrates needs by CO2 assimilation during photosynthesis.
Vitamins are needed when plant cells and tissues are grown in vitro, as some vitamins become limiting factors for growth in vitro. Vitamins required for all cell growth, at concentrations ranging from 0.1 to 10.0 mg/litre. Nicotinic acid and pyroxidic acid are added in 0.1-5.0 mg/litre and 0.1-10.0 mg/litre respectively; both are not essential for cell growth. Myo-inositol is commonly included in many vitamin stock solutions, it is a carbohydrate and not a vitamin, but it has been shown to stimulate cell growth in most species.
Amino acids and other nitrogen supplements are added to further stimulate cell growth as they provide plants cells with an immediately available source of nitrogen. Most commonly used organic nitrogen are L-glutamine, L-asparagine and adenine.