Monday, October 6, 2008

Week 15 SIP/MP Experience

Hi all! Its been a long time since we've met, hope u guys are having fun in your respective attachments ^_^ Well this is my experience for this week

For this post I will be touching up on Carbon (energy source), Vitamins and Amino Acids or Other Nitrogen Supplements.

The carbohydrate that is used in the plant media that I am making is sucrose, theoretically, glucose and fructose can be substituted in some cases. Sucrose concentration of cell media is usually 2-3%. Carbohydrates are important in the media as very few plant cell lines can supply their own carbohydrates needs by CO2 assimilation during photosynthesis.

Vitamins are needed when plant cells and tissues are grown in vitro, as some vitamins become limiting factors for growth in vitro. Vitamins required for all cell growth, at concentrations ranging from 0.1 to 10.0 mg/litre. Nicotinic acid and pyroxidic acid are added in 0.1-5.0 mg/litre and 0.1-10.0 mg/litre respectively; both are not essential for cell growth. Myo-inositol is commonly included in many vitamin stock solutions, it is a carbohydrate and not a vitamin, but it has been shown to stimulate cell growth in most species.

Amino acids and other nitrogen supplements are added to further stimulate cell growth as they provide plants cells with an immediately available source of nitrogen. Most commonly used organic nitrogen are L-glutamine, L-asparagine and adenine.


~immortals~ said...

Hi Johan!!

What are some examples of vitamin that can limit growth in plants?

Why is nicotinic acid and pyroxidic acid added when it is not essential for plant growth?


Fluid collectors said...

hello johan, when is glucose and fructose able to be substituted? and what happens if the sucrose percentage exceeds 2-3%

'Z'h'a'o'R'o'n'g' said...


How does some Vitamins act as limiting factors for growth? "limiting" as in inhibiting?

Haha. I'm a little confused there. If vitamins are required for cell growth, how does the 'limiting factor' come into the picture for growth in vitro?


Tan Zhao Rong

SIP said...

Hi Johan,

Since myo-inositol is a carbohydate, can it be used to substitute sucrose so that at the same time, it also stimulates cell growth?

Ying Chee